Suppression of Tumor Formation and Induction of Natural Killer Cell Activity in BALB/c Nude Mice by Human B7-1 (CD80) Gene Transfer Subcutaneously Injected with Human Hepatocellular Carcinoma Cells (Huh-7) / 대한간학회지
The Korean Journal of Hepatology
; : 124-134, 2003.
Article
in Korean
| WPRIM (Western Pacific)
| ID: wpr-113811
Responsible library:
WPRO
ABSTRACT
BACKGROUND/AIMS:
Immunogene therapy is extensively studied for a therapeutic modality of various cancers. This study was conducted to investigate the efficacy of immunogene therapy using the T-cell costimulatory molecule and human B7-1 (CD80, hB7-1) in an in vivo human hepatocellular carcinoma (HCC) model.METHODS:
The stable HCC cell line expressing hB7-1 gene was established using retroviral vector (Huh-7/hB7-1). Of fourteen BALB/c nude mice, 7 were subcutaneously injected with 2 X 10(6) Huh-7/hB7-1 cells, while the other 7 were injected with 2 X 10(6) Huh-7/mock cells as a control group. After the injection, the mice were observed weekly for three months for subcutaneous tumor formation. Assay for natural killer (NK) cell cytotoxicity and serum IFN-gamma was performed at 1 and 2 weeks after inoculation.RESULTS:
In BALB/c nude mice inoculated with Huh-7/hB7-1 cells, no tumor growth was observed. BALB/c nude mice inoculated with Huh-7/hB7-1 cells showed significantly increased NK cell activities of splenocytes compared with those with Huh-7/mock cells. Serum IFN-gamma was not measurable at 1 week, but significantly increased at 2 weeks after inoculation to the level of 470 pg/ml in BALB/c nude mice with Huh-7/mock cells and 521 pg/ml in BALB/c nude mice with Huh-7/hB7-1.CONCLUSIONS:
Our results demonstrate the in vivo anti-tumor immunity and NK cell activation by transfer of hB7-1 gene into human HCC in xenogeneic BALB/c nude mice model. This approach may provide a tool for the development of immunogene therapies against human malignant tumors.
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Killer Cells, Natural
/
Interferon-gamma
/
Gene Transfer Techniques
/
B7-1 Antigen
/
Cytotoxicity, Immunologic
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Liver Neoplasms, Experimental
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Mice, Inbred BALB C
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Mice, Nude
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Neoplasm Transplantation
Limits:
Animals
/
Humans
Language:
Korean
Journal:
The Korean Journal of Hepatology
Year:
2003
Document type:
Article