Cloning and expression of trypsin-encoding cDNA from Blattella germanica and its possibility as an allergen
The Korean Journal of Parasitology
; : 101-110, 2005.
Article
in English
| WPRIM (Western Pacific)
| ID: wpr-115353
Responsible library:
WPRO
ABSTRACT
In this study, the trypsin gene (bgtryp-1) from the German cockroach, Blattella germanica, was cloned via the immunoscreening of patients with allergies to cockroaches. Nucleotide sequence analysis predicted an 863 bp open reading frame which encodes for 257 amino acids. The deduced amino acid sequence exhibited 42-57% homology with the serine protease from dust mites, and consisted of a conserved catalytic domain (GDSGGPLV). bgtryp-1 was determined by both Northern and Southern analysis to be a 0.9 kb, single-copy gene. SDS-PAGE and Western blotting analyses of the recombinant protein (Bgtryp-1) over-expressed in Escherichia coli revealed that the molecular mass of the expressed protein was 35 kDa, and the expressed protein was capable of reacting with the sera of cockroach allergy patients. We also discussed the possibility that trypsin excreted by the digestive system of the German cockroach not only functions as an allergen, but also may perform a vital role in the activation of PAR-2.
Full text:
Available
Health context:
Neglected Diseases
Health problem:
Neglected Diseases
/
Zoonoses
Database:
WPRIM (Western Pacific)
Main subject:
Recombinant Proteins
/
Molecular Sequence Data
/
Allergens
/
Base Sequence
/
Trypsin
/
Blotting, Western
/
Sequence Alignment
/
Amino Acid Sequence
/
Sequence Homology, Amino Acid
/
Genes, Insect
Limits:
Female
/
Humans
/
Male
Language:
English
Journal:
The Korean Journal of Parasitology
Year:
2005
Document type:
Article