Excretory-secretory antigen is better than crude antigen for the serodiagnosis of clonorchiasis by ELISA
The Korean Journal of Parasitology
; : 35-39, 2003.
Article
in English
| WPRIM (Western Pacific)
| ID: wpr-117984
Responsible library:
WPRO
ABSTRACT
Although stool examination is the standard diagnostic method of clonorchiasis, serodiagnosis by ELISA using crude antigen is now widely used because of its convenience. However, ELISA diagnosis still suffers from cross-reactions, and therefore there is a need to improve the present conventional ELISA. The present study was undertaken to evaluate the diagnostic value of ELISA using excretory-secretory antigen (ESA) instead of crude antigen (CA) of Clonorchis sinensis. The diagnostic sensitivity of ELISA using excretory-secretory antigen was 92.5%, which was higher than that of ELISA using crude Clonorchis sinensis antigen (88.2%). In addition, the specificity of excretory-secretory antigen was found 93.1% while that of crude antigen was 87.8%. In summary, Clonorchis sinensis ESA was found to be a better serodiagnostic antigen than CA for ELISA.
Full text:
Available
Health context:
Neglected Diseases
Health problem:
Neglected Diseases
/
Zoonoses
Database:
WPRIM (Western Pacific)
Main subject:
Enzyme-Linked Immunosorbent Assay
/
Sensitivity and Specificity
/
Clonorchiasis
/
Clonorchis sinensis
/
Antigens, Helminth
Type of study:
Diagnostic study
Limits:
Animals
Language:
English
Journal:
The Korean Journal of Parasitology
Year:
2003
Document type:
Article