Role of nitric oxide and interleukin-1beta on expression of matrix metalloproteinase-2 in TL cell line / 대한산부인과학회잡지
Korean Journal of Obstetrics and Gynecology
; : 2225-2230, 2002.
Article
in Korean
| WPRIM (Western Pacific)
| ID: wpr-118707
Responsible library:
WPRO
ABSTRACT
OBJECTIVE:
We studied to investigate whether nitric oxide (NO) and IL-1beta modulate MMP-2 and MMP-9 using TL cell line obtained from the normal term placenta.METHODS:
After culturing TL cell line for 4 hours, we treated 0.1 mM of SNAP (NO donor) and 50 ng/ml of IL-1beta for 0, 1, 3, 6, and 12 hours, for investigating changes from time. We treated SNAP of 0, 0.01, 0.1, and 0.5 mM for 12 hours and IL-1beta of 0, 1, 10, and 50 ng/ml, for investigating changes from concentration. After extraction of total RNA, we performed reverse transcriptase-polymerase chain reaction (RT-PCR), gelatine zymography and Western blot analysis, for investigating expression of MMP-2 and MMP-9.RESULTS:
MMP-9 was not observed in TL cell line. The expressions of MMP-2 mRNA and protein were gradually increased according to the culture time in SNAP treated group. The expressions of MMP-2 mRNA and protein were gradually increased according to the culture time in IL-1beta treated group. The expression of MMP-2 protein was not more increase in SNAP/IL-1beta-treated group than in IL-1beta treated group. The expression of MMP-2 protein was more reduced in SNAP/hemoglobin treated group than in SNAP treated group. MMP-2 protein activity was only increase in SNAP treated group.CONCLUSION:
These results indicate that NO, rather than IL-1beta, upregulates the MMP-2 in TL cell line and furthermore may influence in the invasive process of trophoblasts.
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Placenta
/
Trophoblasts
/
RNA
/
RNA, Messenger
/
Cell Line
/
Blotting, Western
/
Matrix Metalloproteinase 2
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S-Nitroso-N-Acetylpenicillamine
/
Interleukin-1beta
/
Gelatin
Language:
Korean
Journal:
Korean Journal of Obstetrics and Gynecology
Year:
2002
Document type:
Article