Blood-Brain Barrier Experiments with Clinical Magnetic Resonance Imaging and an Immunohistochemical Study
Journal of Korean Neurosurgical Society
; : 203-209, 2010.
Article
in English
| WPRIM (Western Pacific)
| ID: wpr-126059
Responsible library:
WPRO
ABSTRACT
OBJECTIVE:
The purpose of study was to evaluate the feasibility of brain magnetic resonance (MR) images of the rat obtained using a 1.5T MR machine in several blood-brain barrier (BBB) experiments.METHODS:
Male Sprague-Dawley rats were used. MR images were obtained using a clinical 1.5T MR machine. A microcatheter was introduced via the femoral artery to the carotid artery. Normal saline (group 1, n = 4), clotted autologous blood (group 2, n = 4), triolein emulsion (group 3, n = 4), and oleic acid emulsion (group 4, n = 4) were infused into the carotid artery through a microcatheter. Conventional and diffusion-weighted images, the apparent coefficient map, perfusion-weighted images, and contrast-enhanced MR images were obtained. Brain tissue was obtained and triphenyltetrazolium chloride (TTC) staining was performed in group 2. Fluorescein isothiocyanate (FITC)-labeled dextran images and endothelial barrier antigen (EBA) studies were performed in group 4.RESULTS:
The MR images in group 1 were of good quality. The MR images in group 2 revealed typical findings of acute cerebral infarction. Perfusion defects were noted on the perfusion-weighted images. The MR images in group 3 showed vasogenic edema and contrast enhancement, representing vascular damage. The rats in group 4 had vasogenic edema on the MR images and leakage of dextran on the FITC-labeled dextran image, representing increased vascular permeability. The immune reaction was decreased on the EBA study.CONCLUSION:
Clinical 1.5T MR images using a rat depicted many informative results in the present study. These results can be used in further researches of the BBB using combined clinical MR machines and immunohistochemical examinations.
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Perfusion
/
Tetrazolium Salts
/
Brain
/
Capillary Permeability
/
Triolein
/
Magnetic Resonance Imaging
/
Blood-Brain Barrier
/
Magnetic Resonance Spectroscopy
/
Carotid Arteries
/
Cerebral Infarction
Limits:
Animals
/
Humans
/
Male
Language:
English
Journal:
Journal of Korean Neurosurgical Society
Year:
2010
Document type:
Article