Cyclooxgenase-2 Expression in Chronic Inflammatory Bowel Disease:Comparing of Immunohistochemical Stain to Real-time PCR
Intestinal Research
; : 128-134, 2008.
Article
in Ko
| WPRIM
| ID: wpr-128540
Responsible library:
WPRO
ABSTRACT
BACKGROUND/AIMS: This study compared the results of cyclooxygenase-2 (COX-2) expression in inflammatory bowel disease and tuberculous colitis as evident by immunochemical staining and real time polymerase chain reaction (PCR). METHODS: Patients with ulcerative colitis (n=18), Crohn's disease (n=7), tuberculous colitis (n=7) and 10 normal controls were included. Biopsied colonic mucosa was simultaneously used for immunohistochemical staining and real time PCR. RESULTS: Patients with inflammatory bowel disease and tuberculous colitis showed high COX-2 expression by both methods compared to the normal controls. In Crohn's disease patients, the real time PCR value correlated well staining grade; this correlation was not evident in ulcerative colitis patients. In real time PCR, grossly normal colonic mucosa in ulcerative colitis also showed higher expression of COX-2 than normal mucosa. CONCLUSIONS: Real time PCR value of COX-2 is more representative of inflammation state in inflammatory bowel disease than the value from immunohistochemical staining.
Key words
Full text:
1
Database:
WPRIM
Main subject:
Immunohistochemistry
/
Inflammatory Bowel Diseases
/
Colitis, Ulcerative
/
Crohn Disease
/
Polymerase Chain Reaction
/
Colitis
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Colon
/
Cyclooxygenase 2
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Real-Time Polymerase Chain Reaction
/
Inflammation
Limits:
Humans
Language:
Ko
Journal:
Intestinal Research
Year:
2008
Document type:
Article