Evaluation of reparative dentin formation of ProRoot MTA, Biodentine and BioAggregate using micro-CT and immunohistochemistry
Restorative Dentistry & Endodontics
; : 29-36, 2016.
Article
in English
| WPRIM (Western Pacific)
| ID: wpr-130028
Responsible library:
WPRO
ABSTRACT
OBJECTIVES:
The purpose of this study was to assess the ability of two new calcium silicate-based pulp-capping materials (Biodentine and BioAggregate) to induce healing in a rat pulp injury model and to compare them with mineral trioxide aggregate (MTA). MATERIALS ANDMETHODS:
Eighteen rats were anesthetized, cavities were prepared and the pulp was capped with either of ProRoot MTA, Biodentine, or BioAggregate. The specimens were scanned using a high-resolution micro-computed tomography (micro-CT) system and were prepared and evaluated histologically and immunohistochemically using dentin sialoprotein (DSP).RESULTS:
On micro-CT analysis, the ProRoot MTA and Biodentine groups showed significantly thicker hard tissue formation (p < 0.05). On H&E staining, ProRoot MTA showed complete dentin bridge formation with normal pulpal histology. In the Biodentine and BioAggregate groups, a thick, homogeneous hard tissue barrier was observed. The ProRoot MTA specimens showed strong immunopositive reaction for DSP.CONCLUSIONS:
Our results suggest that calcium silicate-based pulp-capping materials induce favorable effects on reparative processes during vital pulp therapy and that both Biodentine and BioAggregate could be considered as alternatives to ProRoot MTA.
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Immunohistochemistry
/
Calcium
/
Dentin
/
Pemetrexed
Type of study:
Prognostic study
Limits:
Animals
Language:
English
Journal:
Restorative Dentistry & Endodontics
Year:
2016
Document type:
Article