Activated platelets induce secretion of interleukin-1beta, monocyte chemotactic protein-1, and macrophage inflammatory protein-1alpha and surface expression of intercellular adhesion molecule-1 on cultured endothelial cells
Journal of Korean Medical Science
; : 273-278, 2000.
Article
in En
| WPRIM
| ID: wpr-132629
Responsible library:
WPRO
ABSTRACT
Atherosclerosis is an inflammatory disease. Platelet-endothelium interaction plays an important role in the pathophysiology of atherogenesis. We investigated the role of activated platelets for secretion of interleukin (IL)-1beta, monocyte chemotactic protein (MCP)-1 and macrophage inflammatory protein (MIP)-1alpha and expression of intercellular adhesion molecule (ICAM)-1 on endothelial cells. Human umbilical vein endothelial cells (HUVEC) were incubated with non-stimulated or ADP-activated platelets for 6 hr. Secretion of interleukin (IL)-1beta, MCP-1 and MIP-1alpha and surface expression of ICAM-1 were measured by ELISA and flow cytometry. In the presence of activated platelets, the secretion of IL-1beta, MCP-1, and MIP-1alpha and surface expression of ICAM-1 were significantly increased compared with non-activated platelets. The present study shows that activated platelets may contribute to expression of various inflammatory mediators on endothelial cells.
Key words
Full text:
1
Database:
WPRIM
Main subject:
Blood Platelets
/
Endothelium, Vascular
/
Platelet Activation
/
Cells, Cultured
/
Interleukin-1
/
Intercellular Adhesion Molecule-1
/
Chemokine CCL2
/
Macrophage Inflammatory Proteins
Limits:
Humans
Language:
En
Journal:
Journal of Korean Medical Science
Year:
2000
Document type:
Article