IL-13 R110Q, a Naturally Occurring IL-13 Polymorphism, Confers Enhanced Functional Activity in Cultured Human Bronchial Smooth Muscle Cells
Allergy, Asthma & Immunology Research
; : 377-382, 2013.
Article
in English
| WPRIM (Western Pacific)
| ID: wpr-133321
Responsible library:
WPRO
ABSTRACT
PURPOSE:
Interleukin (IL)-13, a Th2-type cytokine, plays a pivotal role in the pathogenesis of asthma through its direct effects on airway smooth muscles. A naturally occurring IL-13 polymorphism, R110Q, is strongly associated with increased total serum IgE levels and asthma. In the present study, we aimed to determine whether the IL-13 R110Q variant would display different biochemical properties or altered functions in comparison with wild-type (WT) IL-13 in cultured human bronchial smooth muscle cells (hBSMCs).METHODS:
Culture supernatants and cell proteins were collected from cultured hBSMCs that were treated with 50 ng/mL IL-13 or IL-13 R110Q for 24 hours. Eotaxin released into hBSMC culture medium was determined by ELISA. The expression levels of the high-affinity IgE receptor (FcepsilonRI) alpha-chain, smooth muscle-specific actin alpha chain (alpha-SMA), smooth muscle myosin heavy chain (SmMHC), and calreticulin in the cells were measured on Western blots.RESULTS:
Compared with WT IL-13, treatment with the IL-13 R110Q variant resulted in a significant increase in eotaxin release as well as significant, although modest, increases in the expression levels of alpha-SMA, SmMHC, calreticulin, and FcepsilonRI alpha-chain.CONCLUSIONS:
The results of the present study suggenst that the IL-13 R110Q variant may enhance enhanced functional activities in hBSMCs.
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Asthma
/
Immunoglobulin E
/
Enzyme-Linked Immunosorbent Assay
/
Proteins
/
Actins
/
Interleukins
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Interleukin-13
/
Myosin Heavy Chains
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Myocytes, Smooth Muscle
/
Calreticulin
Limits:
Humans
Language:
English
Journal:
Allergy, Asthma & Immunology Research
Year:
2013
Document type:
Article