Decreased nitric oxide synthesis in rats with chronic renal failure
Journal of Korean Medical Science
; : 425-430, 2000.
Article
in English
| WPRIM (Western Pacific)
| ID: wpr-135355
Responsible library:
WPRO
ABSTRACT
The present study was aimed at investigating whether an altered role of nitric oxide (NO) is involved in chronic renal failure (CRF). Rats were subjected to 5/6 nephrectomy and kept for 6 weeks to induce CRF. On the experimental day, after measurement of arterial pressure under anesthesia, the arterial blood was collected, and thoracic aorta and kidney were rapidly taken. NO metabolites (NOx) were determined in the plasma, urine, aorta and kidney. The expression of NO synthase (NOS) isozymes was determined in the kidney and aorta by Western blot analysis. The expression of NOS mRNA in the glomeruli was also determined by RT-PCR. There were significant increases in arterial pressure and serum creatinine levels in CRF. Urine NOx levels were decreased in CRF, whereas plasma NOx levels were not altered. Aorta and kidney tissue NOx levels were also decreased in CRF. The expression of endothelial constitutive (ec) and inducible (i) isoforms of NOS proteins was decreased in the kidney and aorta in CRF. Accordingly, the expression of ecNOS and iNOS mRNA was decreased in the glomeruli in CRF. In conclusion, NO synthesis is decreased in the kidney and vasculature of CRF rats.
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Organ Specificity
/
Aorta, Thoracic
/
RNA, Messenger
/
Comparative Study
/
Enzyme Induction
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Rats, Sprague-Dawley
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Nitric Oxide Synthase
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Isoenzymes
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Kidney
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Kidney Failure, Chronic
Limits:
Animals
Language:
English
Journal:
Journal of Korean Medical Science
Year:
2000
Document type:
Article