Oxidative Damage and Nuclear Factor Erythroid 2-Related Factor 2 Protein Expression in Normal Skin and Keloid Tissue
Annals of Dermatology
; : 507-516, 2015.
Article
in English
| WPRIM (Western Pacific)
| ID: wpr-142541
Responsible library:
WPRO
ABSTRACT
BACKGROUND:
Reactive oxygen species (ROS) play an important role in the induction of apoptosis under pathological conditions. Recently, a significant increase in ROS production and disrupted apoptosis mechanisms in keloids have been reported. Nuclear factor erythroid 2-related factor 2 (Nrf2) represents one of the most important cellular defense mechanisms against oxidative stress and is implicated in the regulation of apoptosis. Recently, it has been reported that Nrf2 upregulates Bcl-2, an anti-apoptotic protein.OBJECTIVE:
To compare Nrf2 protein expression in normal skin tissues to keloid tissues.METHODS:
ROS generation in keloid tissues was evaluated with OxyBlot analysis. Western blotting and/or immunohistochemical staining approaches were used to study expression of Nrf2 or Bcl-2 in keloid and normal skin tissues. Cellular fractionation was performed to examine subcellular distribution of Nrf2. Transfection of fibroblasts with Nrf2-specific small interfering RNA (siRNA) was conducted to understand the relationship between Nrf2 expression and apoptosis induction.RESULTS:
Protein oxidation, a marker of oxidative stress, is increased in keloid tissues. Western blot analysis clearly showed that Nrf2 and Bcl-2 are downregulated in keloid tissues. Immunohistochemical staining of Nrf2 confirmed the results of the western blot analysis. Transfection of fibroblasts with the Nrf2-specific siRNA results in increased apoptosis and decreased cell viability.CONCLUSION:
Collectively, our data indicate that Nrf2 expression is downregulated in keloid tissues, and that Nrf2 is involved in the development of apoptosis in Nrf2 siRNA-transfected fibroblasts. We propose that a defective antioxidant system and apoptotic dysregulation may participate in keloid pathogenesis.
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Skin
/
Transfection
/
Cell Survival
/
Blotting, Western
/
Reactive Oxygen Species
/
Apoptosis
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Oxidative Stress
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Defense Mechanisms
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RNA, Small Interfering
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NF-E2-Related Factor 2
Language:
English
Journal:
Annals of Dermatology
Year:
2015
Document type:
Article