Pseudolaric acid B induces apoptosis via activation of c-Jun N-terminal kinase and caspase-3 in HeLa cells
Experimental & Molecular Medicine
; : 551-556, 2004.
Article
in English
| WPRIM (Western Pacific)
| ID: wpr-145925
Responsible library:
WPRO
ABSTRACT
Pseudolaric acid B was isolated from Pseudolarix kaempferi Gordon (Pinaceae) and was evaluated for the anti-cancer effect in HeLa cells. We observed that pseudolaric acid B inhibited cell proliferation and induced apoptosis in a time- and dose-dependent manner. HeLa cells treated with pseudolaric acid B showed typical characteristics of apoptosis including the morphological changes and DNA fragmentation. JNK inhibitor, SP600125, markedly inhibited pseudolaric acid B-induced cell death. In addition, Bcl-2 expression was down-regulated while Bax protein level was up-regulated. Caspase-3 inhibitor, z-DEVD-fmk, partially blocked pseudolaric acid B-induced cell death, and the expression of two classical caspase substrates, PARP and ICAD, were both decreased in a time- dependent manner, indicative of downstream caspase activation.
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Oligopeptides
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HeLa Cells
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Signal Transduction
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Cysteine Proteinase Inhibitors
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Down-Regulation
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Up-Regulation
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Apoptosis
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Proto-Oncogene Proteins c-bcl-2
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Caspases
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JNK Mitogen-Activated Protein Kinases
Limits:
Humans
Language:
English
Journal:
Experimental & Molecular Medicine
Year:
2004
Document type:
Article