Clinical Availability of Component-Resolved Diagnosis Using Microarray Technology in Atopic Dermatitis
Annals of Dermatology
; : 437-446, 2014.
Article
in English
| WPRIM (Western Pacific)
| ID: wpr-146302
Responsible library:
WPRO
ABSTRACT
BACKGROUND:
Various allergens play a role in the elicitation or exacerbation of eczematous skin lesions in atopic dermatitis (AD), and much research effort has been focused on improving diagnostic tests to identify causative allergens.OBJECTIVE:
The purpose of this study was to evaluate the diagnostic effectiveness of a newly introduced microarray-based specific immunoglobulin E detection assay, ImmunoCAP ISAC, for use in AD patients.METHODS:
The serum samples of 25 AD patients were tested by using ISAC and a multiple allergen simultaneous test-enzyme immunoassay (MAST-EIA). In addition, 10 of the 25 patients underwent skin prick testing (SPT). The positive reaction rates to allergens in each test and the agreements, sensitivities, and specificities of ISAC and MAST-EIA were evaluated versus the SPT results.RESULTS:
For ISAC versus SPT, the overall results were as follows sensitivity, 90.0%; specificity, 98.2%; positive predictive value (PPV), 90.0%; and negative predictive value (NPV), 98.2%. The total agreement and kappa value for ISAC versus SPT were 96.9% and 0.882, respectively. For MAST-EIA versus SPT, the sensitivity was 80.0%, specificity 92.7%, PPV 66.7%, and NPV 96.2%. The total agreement and kappa value for MAST-EIA versus SPT were 90.8% and 0.672, respectively. The overall agreement between the ISAC and MAST-EIA results was 88%.CONCLUSION:
The ISAC results in AD correlated well with the SPT results, and compared favorably to the MAST-EIA results. This study demonstrates the potential of ISAC as a convenient allergic diagnostic method in AD patients.
Full text:
Available
Health context:
Sustainable Health Agenda for the Americas
Health problem:
Goal 5: Medicines, vaccines and health technologies
Database:
WPRIM (Western Pacific)
Main subject:
Skin
/
Immunoglobulin E
/
Immunoglobulins
/
Immunoassay
/
Allergens
/
Dermatitis, Atopic
/
Diagnosis
/
Diagnostic Tests, Routine
Type of study:
Diagnostic study
Limits:
Humans
Language:
English
Journal:
Annals of Dermatology
Year:
2014
Document type:
Article