Hydration status affects osteopontin expression in the rat kidney
Journal of Veterinary Science
; : 269-277, 2016.
Article
in English
| WPRIM (Western Pacific)
| ID: wpr-148743
Responsible library:
WPRO
ABSTRACT
Osteopontin (OPN) is a secretory protein that plays an important role in urinary stone formation. Hydration status is associated with the development of urolithiasis. This study was conducted to examine the effects of dehydration and hydration on OPN expression in the rat kidney. Animals were divided into three groups, control, dehydrated, and hydrated. Kidney tissues were processed for light and electron microscope immunocytochemistry, in situ hybridization, and immunoblot analysis. Dehydration induced a significant increase in OPN protein expression, whereas increased fluid intake induced a decrease in protein expression. Under control conditions, OPN protein and mRNA expression were only detected in the descending thin limb (DTL). Dehydration induced increased expression in the DTL and the development of detectable expression in the thick ascending limb (TAL). In contrast, OPN expression levels declined to less than the controls in the DTL after hydration, while no expression of either protein or mRNA was detectable in the TAL. Immunoelectron microscopy demonstrated that hydration status altered tubular ultrastructure and intracellular OPN expression in the Golgi apparatus and secretory cytoplasmic vesicles. These data confirm that changes in oral fluid intake can regulate renal tubular epithelial cell OPN expression.
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
RNA, Messenger
/
Immunohistochemistry
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Urinary Calculi
/
Control Groups
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Microscopy, Immunoelectron
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In Situ Hybridization
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Cytoplasmic Vesicles
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Dehydration
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Epithelial Cells
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Urolithiasis
Limits:
Animals
Language:
English
Journal:
Journal of Veterinary Science
Year:
2016
Document type:
Article