Gastroprotective Effects of PMK-S005 against Ethanol-Induced Acute Gastric Damage in Rats
Gut and Liver
; : 348-355, 2016.
Article
in English
| WPRIM (Western Pacific)
| ID: wpr-155150
Responsible library:
WPRO
ABSTRACT
BACKGROUND/AIMS:
This study aimed to examine the gastroprotective effects of PMK-S005, which is a synthetic S-allyl-L-cysteine (SAC; a sulfur-containing amino acid), against acute ethanol-induced gastric damage in rats.METHODS:
Sprague-Dawley rats were divided into six groups, including a nonethanol group, groups treated with absolute ethanol 1 hour after pretreatment with various doses of PMK-S005 (1, 5, and 10 mg/kg) or rebamipide (50 mg/kg), and an absolute ethanol-only group. Ethanol-induced gross ulcer and mucus levels were measured. Myeloperoxidase, tumor necrosis factor α, interleukin 1β, PGE2, LTB4, cPLA2, COX-1, and COX-2 levels were estimated by enzyme-linked immunosorbent assay or Western blot analysis. Furthermore, the protein expression levels of antioxidant enzymes, including heme oxygenase-1 (HO-1), NAD(P)Hquinine oxidoreductase 1 (NQO-1), GCLC, and GCLM, were assessed.RESULTS:
PMK-S005 significantly attenuated the ethanol-induced gastric damage; it reduced mucosal inflammatory cytokine production and increased mucus levels. The expression levels of cPLA2, COX-1, and COX-2 were decreased by PMK-S005. PMK-S005 did not affect PGE2 synthesis, but LTB4 production was significantly suppressed. In addition, long-term administration of PMK-S005 significantly increased the expression of HO-1, NQO-1, GCLC, and GCLM.CONCLUSIONS:
These results strongly suggest that PMK-S005 prevents gastric mucosal damage and that these gastroprotective activities are due to anti-inflammatory effects and enhancement of the gastric defense system, including antioxidant enzymes.
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Ulcer
/
Enzyme-Linked Immunosorbent Assay
/
Dinoprostone
/
Blotting, Western
/
Interleukins
/
Tumor Necrosis Factor-alpha
/
Rats, Sprague-Dawley
/
Leukotriene B4
/
Peroxidase
/
Ethanol
Limits:
Animals
Language:
English
Journal:
Gut and Liver
Year:
2016
Document type:
Article