Ethanol extract of Angelica gigas inhibits croton oil-induced inflammation by suppressing the cyclooxygenase - prostaglandin pathway
Journal of Veterinary Science
; : 43-50, 2010.
Article
in En
| WPRIM
| ID: wpr-160874
Responsible library:
WPRO
ABSTRACT
The anti-inflammatory effects of an ethanol extract of Angelica gigas (EAG) were investigated in vitro and in vivo using croton oil-induced inflammation models. Croton oil (20 microgram/mL) up-regulated mRNA expression of cyclooxygenase (COX)-I and COX-II in the macrophage cell line, RAW 264.7, resulting in the release of high concentrations of prostaglandin E2 (PGE2). EAG (1~10 microgram/mL) markedly suppressed croton oil-induced COX-II mRNA expression and PGE2 production. Application of croton oil (5% in acetone) to mouse ears caused severe local erythema, edema and vascular leakage, which were significantly attenuated by oral pre-treatment with EAG (50~500 mg/kg). Croton oil dramatically increased blood levels of interleukin (IL)-6 and PGE2 without affecting tumor-necrosis factor (TNF)-alpha and nitric oxide (NO) levels. EAG pre-treatment remarkably lowered IL-6 and PGE2, but did not alter TNF-alpha or NO concentrations. These results indicate that EAG attenuates inflammatory responses in part by blocking the COX-PGE2 pathway. Therefore, EAG could be a promising candidate for the treatment of inflammatory diseases.
Key words
Full text:
1
Database:
WPRIM
Main subject:
RNA, Messenger
/
Plant Extracts
/
Dinoprostone
/
Cell Line
/
Interleukin-6
/
Tumor Necrosis Factor-alpha
/
Plant Roots
/
Reverse Transcriptase Polymerase Chain Reaction
/
Angelica
/
Cyclooxygenase 1
Type of study:
Prognostic_studies
Limits:
Animals
Language:
En
Journal:
Journal of Veterinary Science
Year:
2010
Document type:
Article