Detection of Rotavirus from the Inner Surfaces of Domestic Refrigerators
Laboratory Medicine Online
; : 93-97, 2016.
Article
in Korean
| WPRIM (Western Pacific)
| ID: wpr-16399
Responsible library:
WPRO
ABSTRACT
BACKGROUND:
Rotavirus is the leading cause of acute viral gastroenteritis, particularly in children, and is transmitted through the fecal-to-oral route by contaminated food or the environment. This study examined the contamination of the inner surfaces of domestic refrigerators with pathogens causing gastroenteritis.METHODS:
Swab specimens from shelf surfaces of freezers and refrigerators were collected from 10 domestic refrigerators. Multiplex PCR for bacterial and viral pathogens causing acute gastroenteritis was performed. The VP7 and VP4 genes of rotavirus were amplified and then analyzed by DNA sequencing.RESULTS:
Rotavirus was detected in five domestic refrigerators in the same apartment complex. All rotavirus samples showed the G1 genotype and the same DNA sequences. No pathogens causing acute gastroenteritis were identified in the other five domestic refrigerators.CONCLUSIONS:
The inner surfaces of domestic refrigerators can be contaminated with pathogens causing acute gastroenteritis, such as rotavirus. Attention should be given to the hygiene of refrigerators. To estimate the contamination or hygienic status for food storage, testing for viral pathogens combined with ordinary bacterial cultures may be necessary.
Full text:
Available
Health context:
Neglected Diseases
Health problem:
Diarrhea
/
Neglected Diseases
/
Zoonoses
Database:
WPRIM (Western Pacific)
Main subject:
Base Sequence
/
Hygiene
/
Sequence Analysis, DNA
/
Rotavirus
/
Food Storage
/
Multiplex Polymerase Chain Reaction
/
Foodborne Diseases
/
Gastroenteritis
/
Genotype
Type of study:
Diagnostic study
Limits:
Child
/
Humans
Language:
Korean
Journal:
Laboratory Medicine Online
Year:
2016
Document type:
Article