Analysis of Clinical Characteristics and S Gene Mutation of Hepatitis B Virus (HBV) in Patients with Hepatitis B Surface Antigen RIA Negative and HBV DNA Positive / 대한진단검사의학회지
The Korean Journal of Laboratory Medicine
; : 224-230, 2009.
Article
in Korean
| WPRIM (Western Pacific)
| ID: wpr-166684
Responsible library:
WPRO
ABSTRACT
BACKGROUND:
We investigated hepatitis B virus (HBV) infection cases, who were HBsAg negative by radioimmunoassay (RIA) and HBV DNA positive for their clinical characteristics, the S gene mutation of hepatitis B virus (HBV), and usefulness of other HBsAg immunoassay.METHODS:
Among the patients requested for HBV DNA quantification, 16 patients positive in HBV DNA but negative in HBsAg RIA (BNIBT HBsAg Kit, China) were enrolled. The "a" determinant of HBV S gene was sequenced and clinical characteristics were reviewed. Additional HBsAg assay was performed using Architect HBsAg kit (Abbott laboratories, USA) employing chemiluminescent immunoassay method.RESULTS:
Eleven of the 16 patients showed multiple mutations in the "a" determinant. These patients received liver transplantation several years ago and have been treated with hepatitis B immune globulin (HBIG) and antiviral drugs. G145R mutation was found in 8 patients and G145K, D144G, and D144A were also frequently found. Among 9 of the 11 patients tested for HBsAg by Architect HBsAg kit, 8 showed positive results. Among 4 of the remaining 5 patients, only 2 showed weak positive results (< or =1 IU/mL) in Architect HBsAg kit.CONCLUSIONS:
HBV DNA-positive/HBsAg RIA-negative results were mostly observed in the patients treated with HBIG after liver transplantation, in whom HBIG escape mutations were found. Majority of these cases were positive in Architect HBsAg assay, and it is recommended to use other HBsAg immunoassay methods that are more sensitive than RIA in the detection limit as well as in the detection of escape mutant in hospitals performing liver transplantation.
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Antiviral Agents
/
DNA, Viral
/
Immunoglobulins
/
Immunoassay
/
Radioimmunoassay
/
Molecular Sequence Data
/
Hepatitis B virus
/
Amino Acid Sequence
/
Hepatitis B
/
Hepatitis B Surface Antigens
Limits:
Adolescent
/
Adult
/
Aged
/
Female
/
Humans
/
Male
Language:
Korean
Journal:
The Korean Journal of Laboratory Medicine
Year:
2009
Document type:
Article