Development of Urinary Bladder Pre-Neoplasia by Schistosoma haematobium Eggs and Chemical Carcinogen in Mice
The Korean Journal of Parasitology
; : 21-29, 2017.
Article
in English
| WPRIM (Western Pacific)
| ID: wpr-168709
Responsible library:
WPRO
ABSTRACT
Schistosoma haematobium is a biocarcinogen of human urinary bladder (UB). The present study investigated developing UB cancer mouse model by injecting S. haematobium eggs into the bladder wall and introduction of chemical carcinogens. Histopathological findings showed mild hyperplasia to epithelial vacuolar change, and high grade dysplasia. Squamous metaplasia was observed in the S. haematobium eggs+NDMA group at week 12 but not in other groups. Immunohistochemistry revealed significantly high expression of Ki-67 in urothelial epithelial cells of the S. haematobium eggs+BBN group at week 20. The qRT-PCR showed high expression of p53 gene in S. haematobium eggs group at week 4 and S. haematobium eggs+BBN group at week 20. E-cadherin and vimentin showed contrasting expression in S. haematobium eggs+BBN group. Such inverse expression of E-cadherin and vimentin may indicate epithelial mesenchymal transition in the UB tissue. In conclusion, S. haematobium eggs and nitrosamines may transform UB cells into squamous metaplasia and dysplasia in correlation with increased expression of Ki-67. Marked decrease in E-cadherin and increase in p53 and vimentin expressions may support the transformation. The present study introduces a promising modified animal model for UB cancer study using S. haematobium eggs.
Full text:
Available
Health context:
Neglected Diseases
Health problem:
Neglected Diseases
/
Schistosomiasis
/
Zoonoses
Database:
WPRIM (Western Pacific)
Main subject:
Ovum
/
Schistosoma
/
Schistosoma haematobium
/
Vimentin
/
Urinary Bladder
/
Urinary Bladder Neoplasms
/
Immunohistochemistry
/
Carcinogens
/
Cadherins
/
Genes, p53
Type of study:
Prognostic study
Limits:
Animals
/
Humans
Language:
English
Journal:
The Korean Journal of Parasitology
Year:
2017
Document type:
Article