A Study on the Expression of Proliferating Cell Nuclear Antigen and Apoptosis of the Hepatocellular Carcinoma in Human and Hepatitis B Virus X Transgenic Mice
Korean Journal of Pathology
; : 129-136, 2001.
Article
in Korean
| WPRIM (Western Pacific)
| ID: wpr-173554
Responsible library:
WPRO
ABSTRACT
BACKGROUND:
This experiment was designed to study the cell kinetics of hepatocellular carcinoma (HCC) in both hepatitis B virus X (HBx) transgenic mice and humans.METHODS:
The immunohistochemical stain of proliferating cell nuclear antigen (PCNA) and TdT-mediated dUTP-biotin nick end labeling (TUNEL) assay of apoptosis were used on formalin fixed-paraffin embedded tissues.RESULTS:
PCNA labeling indices (PCNA-LI) in the liver of HBx transgenic mice were markedly increased in HCC (11.3%) compare to the dysplastic areas (1.3%) and in the liver of non-transgenic littermates (0.1%). There was no significant difference of PCNA-LI in the dysplastic areas between HCC developed mice and non-HCC developed mice. Apoptosis labeling indices (Apoptosis-LI) in both the dysplastic areas and HCC of HBx transgenic mice were similar to those of non-transgenic littermates. PCNA-LI was markedly increased in human HCC (28.9%) compare to the background of HCC (2.9%) and the control liver (2.9%). Apoptosis-LI was decreased in human HCC (0.3%) compare to the background of HCC (0.4%) and the control liver (1.0%).Conclusion:
There is a marked increase of cell proliferating activity in human HCC and in HCC of HBx transgenic mice, and there is a decrease of apoptosis in human HCC, but not in HCC of HBx transgenic mice.
Full text:
Available
Health context:
SDG3 - Health and Well-Being
/
SDG3 - Target 3.4 Reduce premature mortality due to noncommunicable diseases
Health problem:
Target 3.3: End transmission of communicable diseases
/
Digestive System Diseases
/
Liver Cancer
Database:
WPRIM (Western Pacific)
Main subject:
Mice, Transgenic
/
Kinetics
/
Hepatitis B virus
/
Apoptosis
/
Carcinoma, Hepatocellular
/
Proliferating Cell Nuclear Antigen
/
Formaldehyde
/
Hepatitis
/
Hepatitis B
/
Liver
Limits:
Animals
/
Humans
Language:
Korean
Journal:
Korean Journal of Pathology
Year:
2001
Document type:
Article