The Inhibitory Effect of Phytoclear-EL1 on Melanogenesis
Annals of Dermatology
; : 369-375, 2009.
Article
in English
| WPRIM (Western Pacific)
| ID: wpr-174310
Responsible library:
WPRO
ABSTRACT
BACKGROUND:
Phytoclear-EL1, an extract from Euphorbia lathyris seeds, has a whitening effect due to inhibition of tyrosinase activity.OBJECTIVE:
The purpose of this study was to investigate the inhibitory effect of phytoclear-EL1 on melanogenesis.METHODS:
Cultured B-16 melanoma cells and 30 human volunteers were used for in vitro and in vivo studies, respectively. Phytoclear-EL1 was added to the cultured B-16 melanoma cells, and applied to UVB-induced hyperpigmented lesions of human volunteers twice daily for 7 weeks. Changes in the number of B-16 melanoma cells, as well as changes in morphology, melanin content, and tyrosinase activity, were measured and then compared with the normal control and the 10(-3)M arbutin groups. Also, the effect of phytoclear-EL1 on UVB-induced hyperpigmented lesions was examined through subjective and objective measurements.RESULTS:
In the in vitro study (p<0.05), the number, melanin content, and tyrosinase activity of cultured B-16 melanoma cells were decreased in the 5microgram/ml phytoclear-EL1 group compared to the control group. On objective assessment with a chromameter, the 0.2% phytoclear-EL1 group had a larger difference in the mean L values before and 7 weeks after applying phytoclear-EL1 as compared to the other groups. On subjective assessment by both the researchers and subjects 7 weeks after applying experimental materials, the 0.2% phytoclear-EL1 group and positive control (3% arbutin) had higher scores than the placebo groups. These results demonstrated that phytoclear-EL1 in vivo and in vitro had an inhibitory effect on melanogenesis.CONCLUSION:
Phytoclear-EL1 may be a candidate extract in the control of hyperpigmentary disorders.
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Arbutin
/
Seeds
/
Monophenol Monooxygenase
/
Euphorbia
/
Human Experimentation
/
Melanins
/
Melanoma
Type of study:
Controlled clinical trial
Language:
English
Journal:
Annals of Dermatology
Year:
2009
Document type:
Article