The Soluble Tumor Necrosis Factor-Alpha Receptor Suppresses Airway Inflammation in a Murine Model of Acute Asthma
Yonsei Medical Journal
; : 569-575, 2009.
Article
in English
| WPRIM (Western Pacific)
| ID: wpr-178603
Responsible library:
WPRO
ABSTRACT
PURPOSE:
Tumor necrosis factor-alpha (TNF-alpha) is a proinflammatory cytokine that has been implicated in many aspects of the airway pathology in asthma. TNF-alpha blocking strategies are now being tried in asthma patients. This study investigated whether TNF-alpha blocking therapy inhibits airway inflammation and airway hyperresponsiveness (AHR) in a mouse model of asthma. We also evaluated the effect of TNF-alpha blocking therapy on cytokine production and adhesion molecule expression. MATERIALS ANDMETHODS:
Ovalbumin (OVA) sensitized BALB/c female mice were exposed to intranasal OVA administration on days 31, 33, 35, and 37. Mice were treated intraperitoneally with soluble TNF-alpha receptor (sTNFR) during the OVA challenge.RESULTS:
There were statistically significant decreases in the numbers of total cell and eosinophil in bronchoalveolar lavage fluid (BALF) in the sTNFR treated group compared with the OVA group. However, sTNFR-treatment did not significantly decrease AHR. Anti-inflammatory effect of sTNFR was accompanied with reduction of T helper 2 cytokine levels including interleukin (IL)-4, IL-5 and IL-13 in BALF and vascular cell adhesion molecule 1 expression in lung tissue.CONCLUSION:
These results suggest that sTNFR treatment can suppress the airway inflammation via regulation of Th2 cytokine production and adhesion molecule expression in bronchial asthma.
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Asthma
/
Bronchi
/
Enzyme-Linked Immunosorbent Assay
/
Bronchoalveolar Lavage Fluid
/
Immunohistochemistry
/
Ovalbumin
/
Blotting, Western
/
Interleukin-4
/
Interleukin-5
/
Tumor Necrosis Factor-alpha
Type of study:
Prognostic study
Limits:
Animals
Language:
English
Journal:
Yonsei Medical Journal
Year:
2009
Document type:
Article