Ovarian cancer related gene targeting with large circular antisense library / 대한산부인과학회지
Korean Journal of Obstetrics and Gynecology
; : 724-733, 2009.
Article
in English
| WPRIM (Western Pacific)
| ID: wpr-193711
Responsible library:
WPRO
ABSTRACT
OBJECTIVE:
The aim of this study is to find out the genes which are related to ovarian cancer cell growth using large circular antisense library.METHODS:
Clones for antisense library were uni-directionally sub-cloned into pBS SK (-) vector. LC-antisense molecules were then purified from the culture supernatants of the bacterial competent cells superinfected with M13K07 helper bacteriophages. The LC-antisense library to 240 unigene clone was constructed and utilized in the identification of genes functionally involved in the growth of ovarian cancer cells.RESULTS:
The 17 numbers out of the 240 numbers of the antisense library exerted a marked inhibitory effect on the growth of SK-OV 3.CONCLUSION:
The putative functional categorization of each gene was then conducted via public databases. These candidates may be used as target genes for drug development or adjuvant of conventional chemotherapeutic drugs.
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Ovarian Neoplasms
/
Bacteriophages
/
Genetic Therapy
/
Clone Cells
/
Gene Targeting
/
Estrone
Language:
English
Journal:
Korean Journal of Obstetrics and Gynecology
Year:
2009
Document type:
Article