Involvement of Oxidative Stress in Cisplatin-Induced Apoptosis in HeLa Cells / 대한산부인과학회잡지
Korean Journal of Obstetrics and Gynecology
; : 2410-2416, 2003.
Article
in Ko
| WPRIM
| ID: wpr-196014
Responsible library:
WPRO
ABSTRACT
OBJECTIVE: To determine whether oxidants are formed as part of the cisplatin-induced apoptotic process, intracellular markers of oxidative stress were examined. METHODS: Apoptotic death of HeLa cells by cisplatin was confirmed by flow cytometry. RESULTS: The pre-treatment with glutathione (GSH) significantly attenuated cisplatin-induced apoptosis through the reduction of reactive oxygen species (ROS) accumulation and diminished caspases-3 and 9 protease activity. Furthermore, z-VAD-fmk, an inhibitor of pan-caspase, effectively inhibited the activation of caspases and prevented apoptosis by cisplatin, although cisplatin-induced ROS generation was not attenuated. CONCLUSION: These data indicate that ROS may play a role as an upstream mediator of caspases. Taken together, our results suggest that oxidative stress mediates cisplatin-induced apoptosis in HeLa cells.
Key words
Full text:
1
Database:
WPRIM
Main subject:
HeLa Cells
/
Cisplatin
/
Oxidants
/
Reactive Oxygen Species
/
Apoptosis
/
Oxidative Stress
/
Caspases
/
Flow Cytometry
/
Glutathione
Limits:
Humans
Language:
Ko
Journal:
Korean Journal of Obstetrics and Gynecology
Year:
2003
Document type:
Article