Anti-Inflammatory Effect of Mangostenone F in Lipopolysaccharide-Stimulated RAW264.7 Macrophages by Suppressing NF-kappaB and MAPK Activation
Biomolecules & Therapeutics
; : 288-294, 2014.
Article
in English
| WPRIM (Western Pacific)
| ID: wpr-199234
Responsible library:
WPRO
ABSTRACT
Mangostenone F (MF) is a natural xanthone isolated from Garcinia mangostana. However, little is known about the biological activities of MF. This study was designed to investigate the anti-inflammatory effect and underlying molecular mechanisms of MF in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages. MF dose-dependently inhibited the production of NO, iNOS, and pro-inflammatory cytokines (TNF-alpha, IL-6, and IL-1beta) in LPS-stimulated RAW264.7 macrophages. Moreover, MF decreased the NF-kappaB luciferase activity and NF-kappaB DNA binding capacity in LPS-stimulated RAW264.7 macrophages. Furthermore, MF suppressed the NF-kappaB activation by inhibiting the degradation of IkappaBalpha and nuclear translocation of p65 subunit of NF-kappaB. In addition, MF attenuated the AP-1 luciferase activity and phosphorylation of ERK, JNK, and p38 MAP kinases. Taken together, these results suggest that the anti-inflammatory effect of MF is associated with the suppression of NO production and iNOS expression through the down-regulation of NF-kappaB activation and MAPK signaling pathway in LPS-stimulated RAW264.7 macrophages.
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Phosphorylation
/
Phosphotransferases
/
DNA
/
Down-Regulation
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Cytokines
/
NF-kappa B
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Interleukin-6
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Transcription Factor AP-1
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Garcinia mangostana
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Luciferases
Language:
English
Journal:
Biomolecules & Therapeutics
Year:
2014
Document type:
Article