Cardioprotective Effects of Minocycline in Rat Myocardiac Cell Culture Induced by Hypoxia / 소아과
Korean Journal of Pediatrics
; : 685-690, 2004.
Article
in Ko
| WPRIM
| ID: wpr-203172
Responsible library:
WPRO
ABSTRACT
PURPOSE: Minocycline appears to reduce the mortality rate of patients with myocardial infarction. Therefore, the cardioprotective effects of minocycline through reduction of apoptosis were studied in a rat myocardiac cell culture induced by hypoxia. METHODS: Cultured cells from the hearts of Sprague-Dawley rats were divided into two sets of groups: normoxia groups treated with 5% O2 and hypoxia groups treated with 1% O2. After several days of incubation, the control groups were not treated with minocycline, while the sample groups were treated with 1 mug/mL of minocycline. The damaged cells were observed under a microscope, while apoptosis was detected using a Tdt-mediated dUTP nick and labeling(TUNEL) assay control-stained with 4'-6'-diamidino-2-phenylindole-2HCl(DAPI). RESULTS: In normoxia, the control group showed a statistically significant difference from the sample group treated with 1 mug/mL of minocycline. In addition, in hypoxia, there was also a statistically significant difference between the two groups. When comparing the normoxia and hypoxia groups to each other, there was a statistically significant difference between the control groups and sample groups. CONCLUSION: Minocycline was found to be protective for heart in normoxia and in hypoxia in the model of rat myocardiac cell culture.
Key words
Full text:
1
Database:
WPRIM
Main subject:
Cells, Cultured
/
Mortality
/
Rats, Sprague-Dawley
/
Apoptosis
/
Cell Culture Techniques
/
Heart
/
Hypoxia
/
Minocycline
/
Myocardial Infarction
Type of study:
Prognostic_studies
Limits:
Animals
/
Humans
Language:
Ko
Journal:
Korean Journal of Pediatrics
Year:
2004
Document type:
Article