Aldehyde dehydrogenase is used by cancer cells for energy metabolism
Experimental & Molecular Medicine
; : e272-2016.
Article
in English
| WPRIM (Western Pacific)
| ID: wpr-210167
Responsible library:
WPRO
ABSTRACT
We found that non-small-cell lung cancer (NSCLC) cells express high levels of multiple aldehyde dehydrogenase (ALDH) isoforms via an informatics analysis of metabolic enzymes in NSCLC and immunohistochemical staining of NSCLC clinical tumor samples. Using a multiple reaction-monitoring mass spectrometry analysis, we found that multiple ALDH isozymes were generally abundant in NSCLC cells compared with their levels in normal IMR-90 human lung cells. As a result of the catalytic reaction mediated by ALDH, NADH is produced as a by-product from the conversion of aldehyde to carboxylic acid. We hypothesized that the NADH produced by ALDH may be a reliable energy source for ATP production in NSCLC. This study revealed that NADH production by ALDH contributes significantly to ATP production in NSCLC. Furthermore, gossypol, a pan-ALDH inhibitor, markedly reduced the level of ATP. Gossypol combined with phenformin synergistically reduced the ATP levels, which efficiently induced cell death following cell cycle arrest.
Full text:
Available
Health context:
SDG3 - Target 3.4 Reduce premature mortality due to noncommunicable diseases
Health problem:
Endocrine System Diseases
/
Other Respiratory Diseases
/
Trachea, Bronchus, Lung Cancers
Database:
WPRIM (Western Pacific)
Main subject:
Phenformin
/
Mass Spectrometry
/
Gossypol
/
Adenosine Triphosphate
/
Cell Death
/
Protein Isoforms
/
Informatics
/
Aldehyde Dehydrogenase
/
Energy Metabolism
/
Cell Cycle Checkpoints
Limits:
Humans
Language:
English
Journal:
Experimental & Molecular Medicine
Year:
2016
Document type:
Article