Preliminary Study of Tissue-Engineered Ileal Conduit Using Poly (epsilon-Caprolactone) (PCL) Nano-Sheet Seeded with Muscle-Derived Stem Cells
Korean Journal of Urology
; : 282-287, 2009.
Article
in Korean
| WPRIM (Western Pacific)
| ID: wpr-218430
Responsible library:
WPRO
ABSTRACT
PURPOSE:
We studied the feasibility and results of a tissue-engineered ileal conduit using a poly (epsilon-caprolactone) (PCL) nano-sheet seeded with muscle-derived stem cells to replace a conventional ileal conduit in rats. MATERIALS ANDMETHODS:
Muscle-derived stem cells were isolated from the gastrocnemius muscle of female Sprague-Dawley rats (200-250 g, n=6) by use of a preplate technique and were cultured on a PCL nano-sheet. The PCL nano-sheet was implanted into the omentum of rats and was then made into a conical shaped conduit. Rats were sacrificed 4 and 8 weeks after implantation, and morphologic changes were assessed by H&E and immunofluorescence staining, including DAPI staining and staining for myogenin and myosin heavy chain (MyHC).RESULTS:
All rats survived until the end of the experiment. A minimal inflammatory reaction was observed around the PCL nano-sheet in the 4 week specimens but was found to be reduced in the 8 week specimen. Muscle bundles were identified at week 4 as well as week 8 after implantation on H&E staining. Around the PCL sheet, immunostaining for both myogenin and MyHC were positive, indicating skeletal muscle differentiation and ingrowth into the PCL sheet.CONCLUSIONS:
A PCL nano-sheet seeded with muscle-derived stem cells showed successful skeletal muscle differentiation at 4 and 8 weeks after implantation. This preliminary result supports the feasibility of a tissue-engineered ileal conduit using a PCL nano-sheet (seeded with muscle-derived stem cells) in place of conventional ileal conduits.
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Omentum
/
Seeds
/
Stem Cells
/
Urinary Diversion
/
Ophthalmoplegia
/
Fluorescent Antibody Technique
/
Rats, Sprague-Dawley
/
Myogenin
/
Muscle, Skeletal
/
Myosin Heavy Chains
Limits:
Animals
/
Female
/
Humans
Language:
Korean
Journal:
Korean Journal of Urology
Year:
2009
Document type:
Article