Neuroprotective effects of geneticin (G418) via apoptosis in perinatal hypoxic-ischemic brain injury / 소아과
Korean Journal of Pediatrics
; : 170-180, 2008.
Article
in English
| WPRIM (Western Pacific)
| ID: wpr-218626
Responsible library:
WPRO
ABSTRACT
PURPOSE:
Some antibiotics were known to exert neuroprotective effects in the animal model of hypoxic-ischemic (H-I) brain injury, but the mechanism is still unclear. A recent study reported that geneticin (G418), an aminoglycoside antibiotic, increased survival of human breast cancer cells by suppressing apoptosis. We investigated the neuroprotective effects of systemically administrated geneticin via anti-apoptosis following the H-I brain injuryMETHODS:
Seven-day-old Sprague-Dawley rat pups were subjected to unilateral (left) common carotid artery occlusion followed by 2.5 hours of hypoxic exposure and the cortical cell culture of rat brain was done under a hypoxic incubator. Apoptosis was measured in the injured hemispheres 7 days after H-I insult and in the injured cells from hypoxic chamber using morphologic analysis by Terminal dUTP Nick-end Labeling(TUNEL) assay and immunohistochemistry for caspase-3, and cytologic analysis by western blot and real time PCR for bax, bcl-2, and caspase-3.RESULTS:
The gross appearance and hematoxylin and eosin stain revealed increased brain volume in the geneticin-treated animal model of perinatal H-I brain injury. The TUNEL assay revealed decreased apoptotic cells after administration of geneticin in the cell culture model of anoxia. Immunohistochemistry showed decreased caspase-3 expression in geneticin-treated cortical cell culture. Western blot and real-time PCR showed decreased caspase-3 expression and decreased ratio of Bax/Bcl-2 expression in geneticin-treated animal model.CONCLUSION:
Geneticin appears to exert a neuroprotective effect against perinatal H-I brain injury at least via anti-apoptosis. However, more experiments are needed in order to demonstrate the usefulness of geneticin as a preventive and rescue treatment for H-I brain injuries of neonatal brain.
Full text:
Available
Health context:
SDG3 - Health and Well-Being
/
SDG3 - Target 3.4 Reduce premature mortality due to noncommunicable diseases
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SDG3 - Target 3.2 Reduce avoidable death in newborns and children under 5
Health problem:
Target 3.2: Reduce avoidable death in newborns and children under 5
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Brain and Nervous System Cancers
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Breast Cancer
/
Infections
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Neonatal Healthcare
Database:
WPRIM (Western Pacific)
Main subject:
Brain
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Brain Injuries
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Breast Neoplasms
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Immunohistochemistry
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Gentamicins
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Blotting, Western
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Carotid Artery, Common
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Apoptosis
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Eosine Yellowish-(YS)
/
Neuroprotective Agents
Limits:
Animals
/
Humans
Language:
English
Journal:
Korean Journal of Pediatrics
Year:
2008
Document type:
Article