Differentiation of rat bone marrow derived Thy-1+ beta2M- cells into hepatocytes / 中华血液学杂志
Chinese Journal of Hematology
; (12): 69-73, 2005.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-229904
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the differentiation of bone marrow derived Thy-1(+)beta(2)M(-) cells (BDTC) into mature and functional liver cells and its mechanism.</p><p><b>METHODS</b>BDTC were cocultured with allyl alcohol (AA)-injured hepatocytes and cultured alone in conditional medium containing HGF and bFGF, respectively. BDTC morphologic transformation was observed with phase-contrast and electron-microscopy. Hepatocyte-specific gene expression in cultured BDTC was identified by immunocytochemistry and reverse transcription-polymerase chain reaction (RT-PCR). Indocyanine green (ICG) ingestion/excretion and urea, albumin production were carried out to evaluate hepatocyte-related function.</p><p><b>RESULTS</b>Some BDTC derived hepatocyte-like cells with high nuclear to cytoplasmic ratio containing mono- or multi-nuclei and abundant mitochondria, endoplasmic reticulum and glycogenic granules appeared after 7-day culture in both the two culture systems. These cells expressed hepatocyte-specific genes (AFP, OV-6, CK18, etc.), and possessed functions of ICG uptake, albumin production and ammonium metabolism.</p><p><b>CONCLUSION</b>Rat BDTCs could differentiate into mature and functional liver cells in special stimulation systems. Moreover, these differentiations were realized by "transdifferentiation", and might dispense with "cell fusion".</p>
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Bone Marrow Cells
/
Immunohistochemistry
/
Alpha-Fetoproteins
/
Gene Expression
/
Cell Differentiation
/
Cells, Cultured
/
Fibroblast Growth Factor 2
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Rats, Wistar
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Hepatocyte Growth Factor
Type of study:
Prognostic study
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Hematology
Year:
2005
Document type:
Article