Genetic diagnosis of polycystic kidney disease using single cell fluorescent polymerase chain reaction / 中国医学科学院学报
Acta Academiae Medicinae Sinicae
; (6): 283-286, 2007.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-229986
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To explore the feasibility of using single cell fluorescent polymerase chain reaction (PCR) in the preimplantation genetic diagnosis (PGD).</p><p><b>METHOD</b>Single buccal cell genetic analysis was performed with fluorescent PCR of linked microsatellite D16S423, followed by electrophoresis on ABI 3730.</p><p><b>RESULT</b>The amplification success rate, allele dropout rate, and diagnostic accuracy rate of the single cell fluorescent PCR were 93.3%, 10.7%, and 89.3%, respectively.</p><p><b>CONCLUSION</b>Single cell fluorescent PCR is a stable and reliable approach for the PGD.</p>
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Pedigree
/
Polymerase Chain Reaction
/
Preimplantation Diagnosis
/
Molecular Diagnostic Techniques
/
Diagnosis
/
Fluorescent Dyes
/
Genetics
/
Polycystic Kidney Diseases
/
Methods
Type of study:
Diagnostic study
Limits:
Female
/
Humans
/
Pregnancy
Language:
Chinese
Journal:
Acta Academiae Medicinae Sinicae
Year:
2007
Document type:
Article