Effects of NKG2D and its ligands RAE-1 and H60 on graft-versus-tumor response / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 160-164, 2007.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-230310
Responsible library:
WPRO
ABSTRACT
The study was purposed to explore the effects of NKG2D receptor and its ligands RAE-1 and H60 on graft-versus-tumor (GVT) response induced by MHC haploidentical bone marrow/spleen cell transplantation. Female (BALB/c x C57BL/6) F1 mice (CB6F1, H-2K(b/d)) inoculated with H22 cells to develop a solid tumor model were the recipients, and bone marrow mixed with spleen cells of the healthy male C57BL/6 (H-2K(b)) mice were the donor cells. GVT response was observed after transplantation that from donor cells T and NK cells were purged with anti-CD3 and anti-NK monoclonal antibody, and the NKG2D receptor was blocked with anti-NKG2D monoclonal antibody, the expression levels of RAE-1 and H60 mRNA in tumor tissue were measured by means of semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) at different time points after transplantation. The results showed that the GVT response of transplantation was reduced after in vitro depletion of T and NK cells or blocking NKG2D receptor in donor cells of the graft, the expression levels of RAE-1 and H60 mRNA in tumor tissue increased after transplantation of haploidential bone marrow mixed with spleen cells. It is concluded that NKG2D and its ligands RAE-1 and H60 may play important roles in GVT response.
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Therapeutics
/
Blood
/
Killer Cells, Natural
/
Receptors, Immunologic
/
Leukemia, Experimental
/
Minor Histocompatibility Antigens
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Hematopoietic Stem Cell Transplantation
/
Graft vs Leukemia Effect
/
Nucleocytoplasmic Transport Proteins
/
Nuclear Matrix-Associated Proteins
Limits:
Animals
Language:
Chinese
Journal:
Journal of Experimental Hematology
Year:
2007
Document type:
Article