Application of quantum dots fluorescent nanoprobes in following the migration of inflammatory cells from local tissue to draining lymph node / 生物医学工程学杂志
Journal of Biomedical Engineering
; (6): 72-76, 2008.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-230652
Responsible library:
WPRO
ABSTRACT
Quantum dots (QDs) have favorable physical and photochemical properties. In this work, we used QDs fluorescent nanoprobes to follow the migration of inflammatory cells from local tissue to draining lymph node in inflammation resolution. Electric pulse stimulation was used to establish inflammation model in mouse tibialis anterior. QDs injected to inflammatory tissue were found to aggregate and endocytosed by inflammatory cells. While in the draining lymph node, QDs mainly distributed in the T cell area. TEM and confocal observation showed that most of QDs in the draining lymph node were located in the endosomes of monocytes/macrophages. Our work shows that QDs can be used as fluorescent probes to follow migration of cells especially phagocytic cells. Thus QDs may be explored in application in immunology research.
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Phagocytes
/
Staining and Labeling
/
Cell Movement
/
Muscle, Skeletal
/
Microscopy, Confocal
/
Quantum Dots
/
Microscopy, Electron, Transmission
/
Nanoparticles
/
Fluorescent Dyes
Limits:
Animals
Language:
Chinese
Journal:
Journal of Biomedical Engineering
Year:
2008
Document type:
Article