Investigation of canine mesenchymal stem cells differentiation to vascular endothelial cell in vitro / 生物医学工程学杂志
Journal of Biomedical Engineering
; (6): 1348-1351, 2007.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-230688
Responsible library:
WPRO
ABSTRACT
To induce endothelial cell, canine bone marrow-derived mesenchymal stem cells (MSCs) were separated from bone marrow by density gradient centrifugation. The isolated MSCs were induced to form endothelial-like cell in the presence of vascular endothelial growth factor (VEGF), endothelial growth factor (EGF) and so on. These results showed that the cells uniformly took on a cobblestone morphology under the light microscope, and cell nucleolus was in the middle of the cells. The cells displayed Weibel-Palade bodies under the transmission electron microscope. vWF, a specific marker of endothelial cell was positive in the cells. The above results demonstrate that MSCs may be differentiated into endothelial cells in vitro.
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Bone Marrow Cells
/
Cell Differentiation
/
Cells, Cultured
/
Endothelial Growth Factors
/
Cell Culture Techniques
/
Cell Biology
/
Endothelial Cells
/
Vascular Endothelial Growth Factor A
/
Mesenchymal Stem Cells
Limits:
Animals
Language:
Chinese
Journal:
Journal of Biomedical Engineering
Year:
2007
Document type:
Article