Activation of transcription factors induced by low concentration of N-methyl-N'-nitro-N-nitrosoguanidine / 浙江大学学报·医学版

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the effect of MNNG on some of the transcription factors such as NF- kappaB, CREB, AP-1 and c-Myc.</p><p><b>METHODS</b>The activities of these transcription factors were measured by transient transfection assay of SEAP vectors.</p><p><b>RESULT</b>The expressions of AP-1, CREB and NF- kappaB driven reporter genes were elevated for about 1.3, 1.4 and 1.3 times in MNNG-treated cells, respectively, as compared to untreated controls. However, the exposure of MNNG had no effect on the activity of c-Myc.</p><p><b>CONCLUSION</b>The activation of certain transcription factors might be involved in the process of untargeted mutation induced by low concentration of MNNG treatment.</p>