Adenoviruses mediated BCL-X1 overexpression protects mice from fulminant hepatic failure / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
; (6): 109-111, 2011.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-231179
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To indentify the relation between hepatic cells apoptosis and the lesion of liver tissue in acute toxic lethal hepatitis.</p><p><b>METHODS</b>60 Wistar mice were randomly divided into normal control, model group and treatment group. Normal control and model group were pretreated by portal vein injection of normal saline, the treatment group was pretreated by portal vein injection of BCL-X1 adenoviruses. The mice of model group and treatment group were received an injection of D-galn and LPS to establish fulminant hepatic failure models 7 days after pretrement. To observe BCL-X1 expression, serum ALT, AST, hepatocyte apoptosis rate, and mortality rate of the three groups.</p><p><b>RESULTS</b>The BCL-X1 expression was higher in treatment group than in model group; 6 hours after fulminant hepatic failure models were established,the serum ALT, AST level of treatment group was lower than model group;The hepatocyte apoptosis rate of treatment group was lower than model group. The death rate of treatment group was lower than model group.</p><p><b>CONCLUSION</b>In fulminant mice hepatic failure models, the hepatocyte apoptosis rate has a positive correlation with death rate, the overexpression of BCL-X1 can decrease the hepatocyte apoptosis rate and the death rate.</p>
Full text:
Available
Health context:
SDG3 - Target 3.4 Reduce premature mortality due to noncommunicable diseases
Health problem:
Digestive System Diseases
Database:
WPRIM (Western Pacific)
Main subject:
Therapeutics
/
Genetic Therapy
/
Gene Expression
/
Adenoviridae
/
Rats, Wistar
/
Liver Failure, Acute
/
Apoptosis
/
Cell Biology
/
Therapeutic Uses
/
Disease Models, Animal
Limits:
Animals
/
Female
/
Humans
Language:
Chinese
Journal:
Chinese Journal of Experimental and Clinical Virology
Year:
2011
Document type:
Article