Drug screening model acting on out-membrane protein OprM in pseudomonas aeruginosa efflux pump system / 中国医学科学院学报
Acta Academiae Medicinae Sinicae
; (6): 359-363, 2004.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-231928
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To establish an efflux pump inhibitor screening model with the out-membrane protein OprM in Pseudomonas aeruginosa efflux pump system as the target point.</p><p><b>METHODS</b>Efflux pump out-membrane protein gene oprM was obtained from standard Pseudomonas aeruginosa PA01 strain. Expression of OprM protein was induced in E. coli strain HS151 with T-easy vector as the cloning vector, and pMMB67EH as the expression vector. In order to evaluate the function of OprM protein, we measured intracellular tetracycline concentrations with liquid scintillation counter, measured the diameters of bacteriostatic circles with paper disc, and then established a screening model accordingly.</p><p><b>RESULTS</b>OprM protein was highly expressed. Using Pseudomonas aeruginosa as the main detecting bacteria, we established a drug screening model acting on OprM. A total of 1 600 microbial fermentation samples were screened with this model, among which 56 positive strains were found, with a positive rate of 3.5%.</p><p><b>CONCLUSION</b>OprM plays an important role in drug efflux. The established model has good specificity and maneuverability.</p>
Full text:
Available
Health context:
Neglected Diseases
Health problem:
Neglected Diseases
/
Zoonoses
Database:
WPRIM (Western Pacific)
Main subject:
Membrane Transport Proteins
/
Pharmacology
/
Plasmids
/
Pseudomonas aeruginosa
/
Bacterial Outer Membrane Proteins
/
Bacterial Proteins
/
Drug Resistance, Microbial
/
Drug Resistance, Multiple
/
Drug Evaluation, Preclinical
/
Escherichia coli
Type of study:
Diagnostic study
/
Screening study
Limits:
Humans
Language:
Chinese
Journal:
Acta Academiae Medicinae Sinicae
Year:
2004
Document type:
Article