PDCD5 induces the apoptosis of human prostate cancer cells PC-3M-1E8 / 中华男科学杂志
National Journal of Andrology
; (12): 979-982, 2007.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-232028
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the apoptosis-promoting effect of PDCD5 on human prostate cancer cells PC-3M-1E8.</p><p><b>METHODS</b>PCI-neo and PCI-neo-PDCD5 were transfected into PC-3M-1E8 cells by Lipofectamine 2000, the viability of the cells was analyzed by MTT assay 16 hours after removal of the serum, and the apoptosis was determined by in situ end-labeling and electron microscopy.</p><p><b>RESULTS</b>The viability and growing speed of the transfected cells were significantly decreased and their apoptotic indexes significantly increased as compared with the control group (P < 0.001).</p><p><b>CONCLUSION</b>PDCD5 may significantly inhibit the in vitro growth and promote the apoptosis of human prostate cancer cells PC-3M-1E8.</p>
Full text:
Available
Health context:
SDG3 - Target 3.4 Reduce premature mortality due to noncommunicable diseases
Health problem:
Prostate Cancer
Database:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Physiology
/
Plasmids
/
Prostatic Neoplasms
/
Transfection
/
Chemistry
/
Apoptosis
/
Reverse Transcriptase Polymerase Chain Reaction
/
In Situ Nick-End Labeling
/
Cell Line, Tumor
Limits:
Humans
/
Male
Language:
Chinese
Journal:
National Journal of Andrology
Year:
2007
Document type:
Article
