Testosterone could induce a rapid rise in intracellular free Ca2+ concentration through binding to the membrane surface of bone marrow-derived macrophages / 中华男科学杂志
National Journal of Andrology
; (12): 784-790, 2007.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-232064
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the ways testosterone influences the murine bone marrow-derived macrophages (BMMs) and how testosterone affects the function of BMMs after bound to their membrane surface.</p><p><b>METHODS</b>BMMs were cultured in vitro, their total RNA and proteins isolated, and the expression of intracellular androgen receptor (AR) detected through RT-PCR and Western blotting. The binding site of testosterone (T) to the membrane surface of BMMs was observed by confocal laser scanning microscopy after T-BSA-FITC incubation. Moreover, the intracellular Ca2+ was tested by Fura-2 method, and the influence of ionic currents on BMMs plasma membrane induced by testosterone was examined by the whole cell patch-clamp.</p><p><b>RESULTS</b>RT-PCR and Western blotting failed to detect intracellular ARs in BMMs, but confocal laser scanning microscopy showed testosterone to be bound to the membrane surface of BMMs by impermeable T-BSA-FITC, inducing a rapid rise in the intracellular free Ca2+ concentration ([Ca2+]i) of Fura-2 loaded BMMs, predominantly due to the influx of extracellular Ca2+ through Ni2+ -blockable Ca2+ channels in the plasma membrane. Similarly, the patch-clamp technique revealed T-induced calcium influx in BMMs.</p><p><b>CONCLUSION</b>It is reasonable to assume that the testosterone receptor exists on the plasma membranes, and testosterone act through unconventional plasma membrane receptors, induce Ca2+ influx and a rapid rise in the intracellular Ca2+ concentration, and influence the function of BMMs.</p>
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Physiology
/
Protein Binding
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Testosterone
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Calcium Channels
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Receptors, Androgen
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Cell Membrane
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Cells, Cultured
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Blotting, Western
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Calcium
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Microscopy, Confocal
Limits:
Animals
Language:
Chinese
Journal:
National Journal of Andrology
Year:
2007
Document type:
Article