Inhibition of telomerase with hTERT antisense enhances TNF-alpha-induced apoptosis in prostate cancer cells PC3 / 中华男科学杂志
National Journal of Andrology
; (12): 723-726, 2007.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-232076
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the inhibiting effect of telomerase with hTERT antisense on TNF-alpha-induced apoptosis in prostate cancer cells PC3.</p><p><b>METHODS</b>Antisense phosphorothioate oligodeoxynucleotide (AS PS-ODN) was synthesized and purified. Telomerase activity was measured by telomeric repeat amplification protocol (TRAP) and telomerase PCR-ELISA Kit, cell viability was determined by MTT assay, and cell apoptosis was observed by morphological method and determined by flow cytometry.</p><p><b>RESULTS</b>AS PS-ODN could significantly inhibit the telomerase activity and increase the susceptibility of TNF-alpha-induced apoptosis of PC3 cells.</p><p><b>CONCLUSION</b>Inhibition of telomerase with hTERT antisense can enhance TNF-alpha-induced apoptosis in prostate cancer cells.</p>
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Pharmacology
/
Prostatic Neoplasms
/
Enzyme-Linked Immunosorbent Assay
/
Cell Survival
/
Polymerase Chain Reaction
/
Tumor Necrosis Factor-alpha
/
Apoptosis
/
Telomerase
/
Oligodeoxyribonucleotides, Antisense
Limits:
Humans
/
Male
Language:
Chinese
Journal:
National Journal of Andrology
Year:
2007
Document type:
Article