Effects of D-limonene on leukemia cells HL-60 and K562 in vitro / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 692-695, 2006.
Article
in Zh
| WPRIM
| ID: wpr-233516
Responsible library:
WPRO
ABSTRACT
To investigate the effects of D-limonene (D-L) on the cell growth and apoptosis in HL-60, K562 cells and to elucidate its mechanism, the influence of D-L on proliferation of HL-60 and K562 cells was determined by propidium iodide assay, the expression levels of mutant p53, bcl-2, bax gene were detected by cell morphological analysis, flow cytometry and immunohistochemistry staining, the D-L-inducing HL-60 and K562 cell apoptosis in vitro was observed systematically. The results showed that D-L inhibited HL-60 and K562 cell growth in a dose- and time-dependent manner with the IC50 of 0.75 mmol/L similarly, D-L induced apoptosis of HL-60 and K562 cells, and expression of bcl-2 gene was down regulated by D-L in a concentration-dependent manner in HL-60 cells. The bcl-2, mutant type of p53 genes were down regulated while bax gene was up regulated by D-L in a concentration-dependent manner in K562 cells. It is concluded that D-L can inhibit proliferation and induce apoptosis of HL-60 and K562 cells. The bcl-2, mutant type of p53 and bax may be involved in the gene regulation of D-L-induced apoptosis.
Full text:
1
Database:
WPRIM
Main subject:
Pharmacology
/
Terpenes
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Tumor Suppressor Protein p53
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Apoptosis
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HL-60 Cells
/
Proto-Oncogene Proteins c-bcl-2
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K562 Cells
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Cell Proliferation
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Bcl-2-Associated X Protein
/
Cyclohexenes
Limits:
Humans
Language:
Zh
Journal:
Journal of Experimental Hematology
Year:
2006
Document type:
Article