Molecular basis of partial D phenotypes in Chinese / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 587-591, 2006.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-233540
Responsible library:
WPRO
ABSTRACT
To investigate the molecular basis of partial D phenotypes in Chinese, D variants with weak D expression was screened by using indirect anti-human globulin test (IAT) method, the polymerase chain reaction-sequence specific primer (PCR-SSP) method was employed to amplify RHD specific exons and their flanking regions. The amplification products were sequenced directly to determine the molecular basis of D variants. The results showed that ten cases of partial D phenotypes, including one case of D Va (Kou.), one case of D Va (Hus.), one case of D Va-like (YH.), and seven cases of D VI type III, were detected from 22 cases of weak D phenotype respectively. All ten cases of partial D phenotypes had one RHD allele deleted. In conclusion, the molecular basis of ten cases of partial D phenotype was confirmed, including D Va (Kou.) and D Va-like (YH.) phenotypes reported firstly in Chinese population.
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Phenotype
/
Rh-Hr Blood-Group System
/
Molecular Sequence Data
/
Base Sequence
/
Asian People
/
Alleles
/
Allergy and Immunology
/
Genetics
/
Mutation
Limits:
Humans
Language:
Chinese
Journal:
Journal of Experimental Hematology
Year:
2006
Document type:
Article