Inhibitory effects of RNA interference on MMP-24 expression and invasiveness of ovarian cancer SKOV(3) cells / 南方医科大学学报
Journal of Southern Medical University
; (12): 781-784, 2009.
Article
in Zh
| WPRIM
| ID: wpr-233684
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the inhibitory effect of RNA interference (RNAi) on MMP-24 expression and invasiveness of ovarian cancer SKOV(3) cells.</p><p><b>METHOD</b>Two pairs of small interfering RNA (siRNA) specific to MMP-24 mRNA were designed and transfected into SKOV(3) cells. RT-PCR and Western blotting were used to detect the mRNA and protein expressions of MMP-24, and the cell invasiveness was assessed using an in vitro invasion test.</p><p><b>RESULTS</b>After transfection with siRNA, the mRNA and protein expression levels of MMP-24 were obviously reduced in SKOV(3) cells, which also showed significantly decreased invasiveness in vitro.</p><p><b>CONCLUSIONS</b>MMP-24 gene silencing by RNAi can suppress the invasiveness of ovarian cancer SKOV(3) cells in vitro, which may provide a new therapeutic approach of ovarian cancer.</p>
Full text:
1
Database:
WPRIM
Main subject:
Ovarian Neoplasms
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Pathology
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Plasmids
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RNA, Messenger
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Transfection
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Gene Expression Regulation, Neoplastic
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Blotting, Western
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RNA Interference
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Cell Line, Tumor
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Matrix Metalloproteinases, Membrane-Associated
Limits:
Female
/
Humans
Language:
Zh
Journal:
Journal of Southern Medical University
Year:
2009
Document type:
Article