Development of an immunochromayography assay method for the detection of Yersinia pestis / 中华流行病学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To develop a method of immunochromatography assay (ICA) with sensitive, specific, rapid, simple and suitable for the detection of Yersinia pestis antigen at the local laboratories.</p><p><b>METHODS</b>Colloidal gold labeled with the anti-F1 antibody of Yersinia pestis, was connnected with the anti-F1 antibody of Yersinia pestis to pyroxylin membrane and assembled them to the dipstick of ICA.</p><p><b>RESULTS</b>Results showed that the rates of sensitivity for F1 antigen and Yersinia pestis were 1 ng/ml and 1.56 x 10(5) CFU/ml respectively. However, Yersinia pseudotuberculosis et al could not be detected by dipstick of ICA.</p><p><b>CONCLUSION</b>The method of ICA appeared to be consistent to those of r-IHA with better specificity and sensitivity but was simple and rapid for the detection of Yersinia pestis and F1 antigen.</p>