GFP used as a reporter system for optimization of K562 cell transfection / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 1174-1176, 2008.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-234274
Responsible library:
WPRO
ABSTRACT
This study was aimed to obtain higher efficiency in gene transfection into K562 cells and to study the role of green fluorescence protein (GFP) as a reporter system. Transfection efficiencies with different methods including electroporation and lipofectamine 2000 transfection, were observed and calculated under fluorescent microscopy by using GFP as a reporter system. The results showed that the transfection efficiency with electroporation (10%) was higher than that with lipofectamine 2000 (1%). In conclusion, the electroporation is a more ideal method for introduction of foreign gene into K562 cells. GFP can be used as a reporter system for optimizing transfection of K562 cells.
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Transfection
/
Genes, Reporter
/
Electroporation
/
K562 Cells
/
Green Fluorescent Proteins
/
Genetics
/
Liposomes
Limits:
Humans
Language:
Chinese
Journal:
Journal of Experimental Hematology
Year:
2008
Document type:
Article