Study on polymorphism of membrane glycoprotein genes related to human platelet alloantigens / 中华医学遗传学杂志
Chinese Journal of Medical Genetics
; (6): 37-41, 2011.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-234322
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the polymorphisms of platelet membrane glycoprotein genes related to human platelet alloantigen (HPA)-1 to 17w.</p><p><b>METHODS</b>The DNA segments of platelet membrane glycoprotein genes related to HPA-1 to 17w were amplified using author's designed primers. The amplification products were purified and directly sequenced to identify the HPA genotype and glycoprotein gene polymorphisms.</p><p><b>RESULTS</b>Thirteen new single nucleotide polymorphisms (SNPs) and a micro-satellite sequence were found in the glycoprotein genes from the 112 random samples, in which two SNPs (1333G/A and 1960G/A) in ITGB3 gene result in two amino acid change (V419M and E628K) on glycoprotein GPIIIa.</p><p><b>CONCLUSION</b>New variants in platelet membrane glycoprotein genes were identified, which may lead to structure change of platelet membrane glycoprotein and affect the accuracy of partial HPA genotyping method.</p>
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Platelet Membrane Glycoproteins
/
Antigens, Human Platelet
/
Polymorphism, Single Nucleotide
/
Genetics
/
Isoantigens
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Medical Genetics
Year:
2011
Document type:
Article