Effects of chain length of polyacrylic acid (PAA) on proteins adsorption of polystyrene-polyacrylic acid (PS-PAA) spherical polyelectrolyte brushes / 生物医学工程学杂志
Journal of Biomedical Engineering
; (6): 421-427, 2013.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-234638
Responsible library:
WPRO
ABSTRACT
We studied the interaction between proteins and polystyrene-polyacrylic acid (PS-PAA) spherical polyelectrolyte brushes with different polyacrylic acid (PAA) chain lengths, including the physical adsorption and chemical adsorption in PBS buffer. Results showed that the amount of bovine serum albumin (BSA) physically adsorbed on PS-PAA spherical polyelectrolyte brushes decreased to a minimum of 33 microg/mg whereas the amount of streptavidin (SA) chemically adsorbed increased with the increase of chain length and carboxyl quantity. The biotin binding capacity of streptavidin chemically adsorbed on PS-PAA spherical polyelectrolyte brushes was roughly evaluated via enzyme competitive inhibition.
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Polyamines
/
Polymers
/
Polystyrenes
/
Surface Properties
/
Acrylic Resins
/
Serum Albumin, Bovine
/
Biosensing Techniques
/
Proteins
/
Chemistry
/
Adsorption
Language:
Chinese
Journal:
Journal of Biomedical Engineering
Year:
2013
Document type:
Article
