Effect of Jinmaitong serum on the proliferation of rat Schwann cells cultured in high glucose medium / 中国结合医学杂志
Chinese journal of integrative medicine
; (12): 293-297, 2008.
Article
in English
| WPRIM (Western Pacific)
| ID: wpr-236248
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of Jinmaitong (JMT) serum on the proliferation of rat Schwann cells (SCs) primarily cultured in high glucose medium.</p><p><b>METHOD</b>SCs were primarily cultured in Dulbecco's minmum essential medium (DMEM control), 50 mmol/L glucose medium (50 mmol/L Glu), 75 mmol/L glucose medium (75 mmol/L Glu), as well as 50 mmol/L glucose medium, with different concentrations of JMT serum (undiluted, 12 diluted and 18 diluted) and Neurotropin (Ntp), respectively. The proliferation of SCs under different conditions was detected by MTT.</p><p><b>RESULT</b>SCs grew exuberantly in DMEM within 24-72 h, but slowed down at 96 h. The proliferation of SCs was inhibited in 50 mmol/L Glu and 75 mmol/L Glu after cultures of 48, 72 and 96 h, which showed that both were significantly different compared to the control group (P<0.01). The inhibition was more significant in 75 mmol/L Glu than in 50 mmol/L Glu (P<0.05). Spearman's rho analysis revealed that the proliferation of SCs had a negative correlation with the concentration of glucose (r=-0.471, P<0.01). Excluding the time factor, partial correlation showed similar results (r=-0.679, P<0.01). After 48 h, the proliferation of SCs increased significantly in JMT12 and Ntp compared with 50 mmol/L Glu (control 0.437+or-0.019, 50 mmol/L Glu 0.367+or-0.035, JMT12 0.426+or-0.024, Ntp 0.422+or-0.013; P<0.01), and there were no statistically significant differences among the JMT groups, the Ntp group and the control group (P>0.05).</p><p><b>CONCLUSIONS</b>The proliferation of SCs was inhibited in high glucose medium, and the inhibition was reduced by different concentrations of JMT serum, especially at JMT12.</p>
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Schwann Cells
/
Drugs, Chinese Herbal
/
Cell Division
/
Cells, Cultured
/
Culture Media
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Cell Biology
/
Glucose
/
Metabolism
Limits:
Animals
Language:
English
Journal:
Chinese journal of integrative medicine
Year:
2008
Document type:
Article