Effects of daidzein on sperm quality, testis gain and testosterone in mice / 中华男科学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To evaluate the effects of daidzein on sperm quality of male mice.</p><p><b>METHODS</b>Three different doses of daidzein were supplemented to pubertal male mice for 21 days(5 mg/kg, 100 mg/kg, 1,000 mg/kg ration). The viability of sperm was determined by eosin-Y, the acrosome was observed by Wright-Giemsa staining, and the testosterone was measured by radioimmunoassay.</p><p><b>RESULTS</b>Daidzein at the dose of 5 mg/kg ration significantly increased serum testosterone levels (P < 0.01), prompted testis gain (P < 0.05), and improved spermatozoa quality. Daidzein at dose of 1,000 mg/kg ration could inhibit the secretion of serum testosterone (P < 0.01), without significant variation in spermatozoa quality. Daidzein at dose of 100 mg/kg ration did not significantly affect sperm quality and other index.</p><p><b>CONCLUSIONS</b>Daidzein can affect sperm quality and in dosage-dependant ways.</p>