A study on the endothelialization mechanism of left ventricular pump / 生物医学工程学杂志

ABSTRACT

The cultural model of porcine aortic endothelial cell (EC) was set up. By combined use of basic fibroblast growth factor(bFGF) with extracellular matrix (ECM), the endothelialization on PU under static culture was accomplished and the attachment rate as well as the morphological changes of EC under flowing shear stress condition were observed when LVAP was working at normal blood circulation pressure (12 KPa). The result revealed that EC treated with bFGF grew very well on the PU material precoated with fibronectin and collagen IV. Under shear stress condition for 1 and 2 hours, the percentage of cell attachment attained 74.42% and 59.7%. The general morphological changes of the cell were not obvious under the scanning electron microscope, slight shrink and cell death or loss were found. This work was done to find out the condition and method of experiment for the accomplishment of endothelialization under the flowing shear stress in the cavity of LVAP.